Carbohydrate metabolism

Q.1-Which of the following enzymes is Thiamine dependent and essential for glucose oxidation in the brain?

a) Pyruvate dehydrogenase complex                

b) Acetyl co A carboxylase

c) Transaldolase                          

d) Succinyl-co A Thiokinase                                         (     a    )



Q.2- The reaction catalyzed by phosphofructo kinase is -

 a) Inhibited by Fructose 2,6 bisphosphate                  

b) Activated by high concentration of ATP

c) A regulatory step of glycolysis  

d) All of the above                                                      (      c   )



Q.3- Pyruvate carboxylase requires ————— as a coenzyme.

a) FMN                              

b) Biotin

c) NAD+

d) TPP                                                                        (    b     )



Q.4- In erythrocytes the most abundantly found Phospho ester is-

 a) Glucose-6 phosphate                

b) Fructose 1, 6 bisphosphate

c) Fructose-6- phosphate

d) 2, 3 bisphosphoglycerate                                        (    d     )



Q.5- Formation of Galactose-1-P from Galactose is catalyzed by-

a) Hexokinase                            

b) Aldolase    

c) Galactokinase                          

d) Galactose-1-P Uridyl transferase                               (     c    )



Q.6- All the following enzymes except one require NADP+ as a coenzyme-

 a) Glucose- 6-phosphate dehydrogenase          

b) Cytosolic Isocitrate dehydrogenase

c) Malic enzyme      

d) Glucose-6-phophatase                                               (     d    )



Q.7- Which of the following product of Triglyceride breakdown and subsequent beta oxidation would undergo gluconeogenesis-

a) Propionyl Co A                        

b) Acetyl CoA

c) Aceto acetate                          

d) Beta hydroxy butyrate                                                 (     a    )



Q.8 – Which of the following reactions generates ATP?

 a) Glucose to Glucose -6-phosphate        

b) Pyruvate to Lactate

c) Phosphoenol pyruvate to Pyruvate      

d) Glucose- 6 phosphate to fructose-6 phosphate             (   c      )



Q.9- Glucose enters muscle cells mostly by:

 a) Simple diffusion

b) Facilitated diffusion using a specific glucose transporter

c) Co-transport with sodium

d) Co-transport with amino acids                                      (     b    )



Q.10- Which one of the following compounds is common to both the oxidative phase and the non- oxidative phase of the pentose phosphate pathway?

 a) Xylulose-5- phosphate                        

b) Glucose-6-phosphate

c) Ribulose-5-phosphate                        

d) Glyceraldehyde-3- phosphate                                        (      a     )



Q.11- Mc Ardle’s syndrome causes muscle cramps and muscle fatigue with increased muscle glycogen. Which of the following enzyme is deficient?

 a) Hepatic hexokinase                            

b) Muscle Phosphorylase

c) Muscle Debranching enzyme              

d) Muscle Hexokinase                                                         (    b     )



Q.12- During starvation, the major source of blood glucose is-

 a) Hepatic Glycogenolysis                      

b) Gluconeogenesis

c)  Muscle Glycogenolysis                      

d) Dietary glucose from intestine                                          (    b     )



Q.13- Hexokinase has a considerably lower km for-

 a) Glucose                                  

b) Fructose

c) Galactose

d) Mannose                                                                          (     a    )



Q.14- In the fasting state there is considerable release of———–from the muscles.

 a) Glycine                                    

b) Alanine      

c) Valine

d) Glutamic acid                                                                  (     b    )



Q.15- A galactosemic child will manifest all except-

 a) Hepatomegaly                          

b) Splenomegaly

c) Cataract

d) Mental retardation                                                            (     b    )



Q16- – Which one of the following reactions is unique to gluconeogenesis-?

a) Lactate<———–>Pyruvate

b) Oxaloacetate—->Phosphoenol pyruvate

c) Glucose-6-phosphate—– >Glucose

d) Phosphoenol pyruvate–>Pyruvate                                    (      b   )



Q.17- The main stores of glycogen are found in—

 a) Adipose tissues    

b) Skeletal muscles            

c) Brain                            

d) Erythrocytes                                                                    (     b    )



Q.18- Succinyl co A is cleaved by-

 a) Succinate dehydrogenase          

b) Succinate Thiokinase

c) Succinate lyase                        

d) Succinate Thioesterase                                                     (    b     )



Q.19- Arsenate binds to-

 a)  Free amino end of enzyme                

b) SH linkages

c) Free carboxyl end

d) Any of the above                                                               (     b    )



Q.20- All are intermediates of glycolysis except-

a) Glucose-6 phosphate                

b) Fructose 1, 6 bisphosphate

c) Fructose-6- phosphate              

d) Glycerol – 3- phosphate                                                    (     d    )



Q.21- The key regulatory enzyme of HMP pathway is-

 a) Glucose- 6-phosphate dehydrogenase

b) Transaldolase

c) Transketolase                          

d) Glucose-6-phophatase                                                       (     a    )



Q.22- The net production of ATP in glycolysis during anaerobic condition is:

 a) 2 from glucose and 3 from glycogen

b) 2 from glucose and 4 from glycogen

c) 3 from glucose and 4 from glycogen

d) 3 from glucose and 2 from glycogen                                   (   a      )



Q.23- Which of the followings does not generate free glucose during the enzymatic breakdown of glycogen in skeletal muscles?

 a) Phosphorylase                        

b) α-1-6-amyloglucosidase  

c) Debranching enzyme      

d) glucose-6-phosphatase                                                          (     a    )



Q.24- Which of the followings promotes glucose and amino acid uptake by muscle?

a) Adrenaline                              

b) Insulin

c) Glucagon                                

d) Cortisol                                                                                   (     b    )



Q.25- In a tissue that metabolizes glucose via the pentose phosphate pathway, C-1 of glucose would be expected to end up principally in:

a) Carbon dioxide                                  

b) Glycogen

c) Phosphoglycerate                              

d) Pyruvate                                                                                   (   a      )




------------------------------------------ Best Wishes: Dr.Ehab Aboueladab, Tel:01007834123 Email:ehab10f@gmail.com,ehababoueladab@yahoo.com ------------------------------------------

Multiple Choice Questions- Carbohydrate metabolism

Q.1 – Which of the following substrates derived from adipose tissues contributes to net Gluconeogenesis in mammalian liver?

a) Alanine

b) Glutamate

c) Glycerol

d) Pyruvate                                                                                                               (    c     )

Q.2 – Which of the following statements is incorrect?

a) Aerobically, oxidative decarboxylation of pyruvate forms acetate that enters the citric acid cycle.

b) In anaerobic muscle, pyruvate is converted to lactate.

c) Reduction of pyruvate to lactate generates a coenzyme essential for Glycolysis.

d) Under anaerobic conditions pyruvate does not form because Glycolysis does not occur.

                                                                                                                                                         (     d    )

Q.3- The steps of Glycolysis between glyceraldehyde 3-phosphate and 3-phosphoglycerate involve all of the following except:

a) ATP synthesis.

b) Catalysis by phosphoglycerate kinase.

c) Oxidation of NADH to NAD+.

d) The formation of 1, 3- bisphosphoglycerate.                                                    (    c     )

Q.4 – The oxidation of 3 mol of glucose by the pentose phosphate pathway may result in the production of:

 a) 2 mol of pentose, 4 mol of NADPH, and 8mol of CO2.

b) 3 mol of pentose, 4 mol of NADPH, and 3mol of CO2.

c) 3 mol of pentose, 6 mol of NADPH, and 3mol of CO2.

d) 4 mol of pentose, 3 mol of NADPH, and 3mol of CO2.                                    (     c    )

Q.5 – How many ATP molecules can be derived from each molecule of acetyl CoA that enters the Krebs’ Cycle?

a) 6

b) 12

c) 18

d) 38                                                                                                                                       (     b    )

Q.6 – All of the following vitamins except one participate in the TCA cycle-

a)Pantothenic acid

b)Lipoic acid

c) Folic acid

d)Riboflavin                                                                                                                        (    c     )

Q.7 – Why Phosphofructokinase rather than hexokinase is the pacemaker of Glycolysis?

a) Glucose 6-phosphate is not solely a glycolytic intermediate

b) Hexokinase has low km for glucose

c) Hexokinase is inhibited by feed back inhibition

d) None of the above                                                                                                       (     a    )

Q.8 - All are correct about pyruvate dehydrogenase complex except one-

a)The formation of acetyl CoA from pyruvate is an irreversible step

b) Pyruvate dehydrogenase is switched off when Acetyl co A is in excess

c)Phosphorylation switches off the activity of the complex.

d) Pyruvate as well as ADP (a signal of low energy charge) inhibits the complex.    (     d    )

Q.9-Cellular isozymes of pyruvate kinase are allosterically inhibited by:

a) High concentrations of AMP.

b) High concentrations of ATP.

c) High concentrations ofFr1,6 bisphosphate.

d) Low concentrations of acetyl-CoA.                                                                       (    b     )

Q.10 – Which of the followings is not an intermediate of the citric acid cycle?

a) Acetoacetate

b) Citrate

c) Oxalosuccinate

d) Succinyl-CoA                                                                                                                 (    a     )

Q.11 - In an anaerobic system that is metabolizing glucose as a substrate, which of the following compounds would you expect to increase in concentration following the addition of fluoride?

 a) 2-phosphoglycerate

b) Glucose

c) Phosphoenolpyruvate

d) Pyruvate                                                                                                                            (   a    )

Q.12 – Which of the following is a coenzyme  in the reaction catalyzed by glyceraldehyde 3-phosphate dehydrogenase?

a) ATP

b) Cu2+

c) Heme

d) NAD+                                                                                                                               (     d    )

Q.13 - An enzyme used in both glycolysis and gluconeogenesis is:

a) 3-phosphoglycerate kinase.

b)Glucose 6-phosphatase.

C) Hexokinase.

d)Phosphofructokinase-1.                                                                                            (     a    )

Q.14 – Which of the following statements about the pentose phosphate pathway is incorrect?

 a) It generates 36 mol of ATP per mole of glucose consumed.

b) It generates 6 moles of CO2for each mole of glucose consumed

c) It is a reductive pathway; it consumes NADH.

d) It provides precursors for the synthesis of nucleotides                                 (     c    )

Q.15 – Which of the following enzymes catalyzes a reaction that involves a decarboxylation reaction?

 a)Pyruvate dehydrogenase

b) Isocitrate dehydrogenase

c) α-keto glutarate dehydrogenase

d all of the above                                                                                                              (     d    )

Q.16 -Anaplerotic reactions are those that result in replenishing intermediates in the TCA cycle. Which of the following enzymes catalyzes an Anaplerotic reaction?

a)  Malate dehydrogenase

b) Pyruvate carboxylase

c) Pyruvate kinase

d). Citrate synthase                                                                                                         (      b   )

Q.17- Which statement BEST describes the fate of Propionyl-CoA in mammalian systems?

a) Propionyl-CoA is metabolized via are action sequence that involves vitamin B12 and biotin.

b) Propionyl-CoA is converted to acetyl CoA.

c) Propionyl-CoA and acetyl CoA condense to form a 5 carbon precursor of a TCA cycle intermediate.

d) Propionyl-CoA is oxidized to Malonate and CoA                                              (      a   )

Q.18 – It is very important to feed the baby very soon after birth, because during the first few hours after birth the enzyme Phosphoenolpyruvate carboxykinase is present in very low amounts, and this fact compromises:

a) Gluconeogenesis

b) Glucose phosphorylation

c) Glycogenesis

d) Glycogenolysis                                                                                                                (    a     )

Q.19 – The citric acid cycle is inhibited by which of the following?

 a) Fluoroacetate

b) Aerobic conditions

c) Malic acid

d) Fluorouracil                                                                                                                    (     a    )

Q.20 – Which of the following enzymes is associated with Glycogen synthesis?

a) Amylo- (1, 4->1,6) – transglycosylase

b) Phosphorylase

c) Amylo-1,6-glucosidase

d) Glucose-6- phosphatase                                                                                            (       a  )

Q.21 – A medical student developed hemolytic anemia after taking the oxidizing Antimalarial drug primaquine. This severe reaction is most likely due to

 a) Glucose-6- phosphate dehydrogenase deficiency

b) Concomitant Scurvy

c) Diabetes

d) Glycogen phosphorylase deficiency                                                                     (    a     )

Q.22- Which of the following explains why individuals with hyperlipidemia should minimize their intake of sucrose and high fructose syrup?

 a) Fructose metabolism is faster than glucose

b) After initial modification fructose is cleaved by a specific Enolase

c) Fructose is ultimately converted to galactose

d) Fructose can be phosphorylated by hexokinase in adipose cells                   (    a     )

Q.23-The major glycolytic product produced under normal circumstances by erythrocytes required for unloading of oxygen to the peripheral tissues is

 a) 2,3bisphosphoglycerate

b) 1,3bisphosphoglycerate

d)Lactate                                                                                                                                       (     a    )

c) Pyruvate

Q.24 – Poorly perfused areas exposed to chronic hypoxia have decreased metabolic energy for tissue maintenance and repair an important reason for this is-

 a)  Increased hexokinase activity owing to increased oxidative phosphorylation

b) Decreased ATP production and increased glucose utilization due to an aerobic mode of glycolysis

c) Increased glucose utilization by HMP pathway on changing from anaerobic to aerobic glycolysis

d) Decreased respiratory quotient on changing from carbohydrates to fats as the major fuel                                                                                                                                                              (    b     )

Q.25 – Asians and Native Americans may flush and feel ill after drinking a small amount of ethanol in alcoholic beverages. This reaction is due to genetic variation in an enzyme that metabolizes the liver metabolite of alcohol, which is-

 a) Methanol

b) Acetone

c) Acetaldehyde

d) Glycerol                                                                                                                                    (    c     )

Q.26 – Which one of the following enzymes catalyzes the phosphorylation of the substrate with the use of inorganic phosphate-?

a) Hexokinase

b) Phospho fructokinase

c) Glyceraldehyde-3-phosphate dehydrogenase

d) Phospho glycerate kinase                                                                                                  (     c    )

------------------------------------------ Best Wishes: Dr.Ehab Aboueladab, Tel:01007834123 Email:ehab10f@gmail.com,ehababoueladab@yahoo.com ------------------------------------------

glycolysis

1) Which of these enzyme reactions is not irreversible in glycolysis?

a) Hexokinase

b) Glucokinase

c) 3-phosphoglycerate kinase

d) Phosphofructokinase-1

e) Pyruvate kinase

2) Which out of the following statements is not true about aerobic glycolysis ?

a) The rate limiting enzyme is phosphofructokinase-1 which converts fructose-6- phosphate to fructose-1,6-bisphosphate

b) The pathway is inhibited allosterically by fructose-2,6-bisphosphate and AMP

c) The pathway takes place in mitochondria of every cell

d) The pathway produces Pyruvate and NADH

e) The pathway begins with glucokinase or hexokinase

3) The first step in the glycolytic pathway in Liver -

a) Is catalyzed by glucokinase

b) Uses ATP and glucose as substrates

c) Produces glucose-6-phosphate and ADP

d) Is an irreversible step

e) All of the above.

4) Phosphoglycerate kinase functions in carbohydrate metabolism to produce ATP via:

a) Oxidative phosphorylation.

b) Substrate level phosphorylation.

c) Oxidative decarboxylation.

d) Phosphorolysis

e) Oxidative deamination

5). The phosphofructokinase-2 enzyme that transfers a phosphate group to fructose-6-phosphate in glycolysis-

a) Is active in the phosphorylated form

b) Catalyzes a reversible reaction

c) Produces fructose-2,6-bisphosphate as a product

d) Is the rate limiting enzyme for glycolysis

e) Produces ATP as a product.

6). The enzyme that produces NADH from a triose phosphate in the glycolytic pathway-

a) Uses NAD+ and dihydroxyacetone phosphate as substrates

b)Produces 3-phosphoglycerate and NADH

c) Is reversible

d) Is called 3-phosphoglycerate kinase

e) Uses FADH2 and glyceraldehyde-3-phosphate as substrates

7). The enzyme that catalyzes first substrate level phosphorylation in glycolysis-

a) Produces 3-phosphoglycerate as a product

b) Produces ADP from AMP

c) Is called glyceraldehyde-3-phosphate dehydrogenase

d) Is called phosphofructokinase

e) Is the rate limiting enzyme of glycolysis.

8). The enzyme that catalyzes the second substrate level phosphorylation of glycolysis-

a) Is called phosphoglyceromutase

b) Produces lactate as a product

c) Uses phosphoenolpyruvate as a substrate

d) Catalyzes a reversible reaction

e) Is stimulated by Glucagon.

9). Which of the following statements about the Lactate dehydrogenase (LDH) reaction is false

a) The enzyme converts pyruvate to lactate

b)The enzyme converts NADH to NAD+

c) The reaction is reversible

d) It is the last enzyme reaction in glycolysis

e) The enzyme is highly active under aerobic conditions

10). The two major factors determining whether a cell oxidizes glucose by aerobic glycolysis or by anaerobic glycolysis are-

a) FADH2 and the number of mitochondria

b) NADH and the ATP/ADP ratio

c) Ca++ and AMP

d) Oxygen pressure and the number of mitochondria

e) Presence of low Glucose and high AMP

11). When glucose is converted to lactate by anaerobic glycolysis, the equivalent of ________ ATPs are derived.  When glucose is completely oxidized to CO2 by glycolysis, Pyruvate dehydrogenase, and the TCA cycle, the equivalent of _______ ATPs are derived.

a) 7;  20

b) 2;  38

c) 7;  32

d)2;  12

e) 7;  25

12).Choose the incorrect statement about the condition ‘When one molecule of glucose is oxidized to 2 molecules of lactate during anaerobic glycolysis’-

a) One molecule of ATP is used by the phosphofructokinase-1 reaction

b)One molecule of ATP is used by either the glucokinase or hexokinase reaction

c) Two molecules of ATP are produced by the Glyceraldehyde -3-P dehydrogenase reaction

d) Two molecules of ATP are produced by the pyruvate kinase reaction

e) No ATP is produced at the level of lactate dehydrogenase reaction.

13). All of the following help to explain some cases of Lactic Acidosis except-

a) High altitude

b) Heart failure

c) Not enough oxygen to satisfy the needs of oxidative phosphorylation

d) Congenital deficiency of liver lactate dehydrogenase

e) Inhibition of the electron transport chain

14) . All of the following are part of the Cori Cycle except-

a) Lactate is the result of anaerobic glycolysis in a number of tissues

b) Lactate is transported to the liver

c) Lactate is converted to glucose in the liver

d) ATP is produced by the conversion of lactate to glucose

e) Glucose is transported from liver to other tissues

15). The ATP/AMP ratio has a major effect upon the rate of ATP production by glycolysis.  ATP and AMP bind to allosteric sites on-

a) Hexokinase

b) Glucokinase

c) Phosphofructokinase-1

d) Phosphofructokinase-2

e) 3-phosphoglycerate kinase

16). Which of the following statements about pyruvate kinase is false -

a) Can convert Phosphoenol pyruvate to pyruvate

b) Is regulated by feed forward regulation

c) Is active in the dephosphorylated form

d) Is also involved in gluconeogenesis

e) Deficiency causes haemolytic anemia.

17). Pasteur effect is due to inhibition of glycolysis by high concentration of -

a) Creatine phosphate

b) ATP

c) ADP

d) AMP

e) Pi

18). In the liver, glucagon causes a decrease in the glycolytic pathway by all of the following mechanisms  except-

a) Increased binding of GTP to G-protein

b) Activation of the cAMP cascade

c) Increased phosphorylation of enzymes by protein kinase A

d) Activation of fructose-2,6-bisphosphatase and inhibition of phosphofructokinase-2

e) Increased binding of fructose-2,6-bisphosphate to phosphofructokinase-1

19). An increased concentration of insulin after meals causes an increase in the rate of  glycolytic pathway by all of the following mechanisms except-

a) Increase uptake of glucose in adipose tissue and muscle

b) Inactivation of the cAMP cascade

c) Increased  dephosphorylation of enzymes activated by protein kinase A

d) Activation of fructose-2,6-bisphosphatase and inhibition of phosphofructokinase-2

e) Induction of Glucokinase and PFK-1

20).During fasting state, the insulin to glucagon ratio drops and all of the following occur except-

a) Protein kinase A will phosphorylate pyruvate kinase

b) The pyruvate kinase step will be activated

c) Protein kinase A will phosphorylate phosphofructokinase-2/fructose-2,6-bisphosphatase

d) The concentration of fructose-2,6-bisphosphate will decrease

e)The liver will not be able to synthesize pyruvate from phosphoenolpyruvate.

21) A high concentration of Glucose-6-P  is inhibitory to which of the following enzymes ?

a) Hexokinase

b) Glucokinase

c) PFK-1

d) PFK-2

e) All of the above,





Answers-       1)-c,               2)- b,              c)-e,               4)-b,               5)-c,               6)-b,                        

                     

                       7)-a,               8)- c,              9)-e,               10)-b,                        11)-b,                        12)-c

                     

                        13)-d              14)-d              15)-c              16)-d              17)-b              18)-e

                     

                        19)-d              20)-b              21)-a



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Glycolysis

1). A patient has chronic obstructive pulmonary disease, enough oxygen is not reaching her tissues.  All of the followings are  expected except-

a) The electron transport chain would be inhibited

b) Glycolysis would be activated by a low ATP/ADP ratio

c) More than normal amounts of ATP would be synthesized .

d) Concentrations of NADH and pyruvate would be lower than normal

e) More than normal amounts of lactate would be produced

2). A 45- year- old woman is diagnosed with breast cancer. The oncologist orders a positron emission tomography(PET) scan of the head to rule out metastasis. This imaging modality covalently links a radioactive isotope most commonly to Glucose to appreciate highly active areas in the body such as tumor. Which of the following traps the tracer in the cell ?

a) Insulin

b) GLUT-1

c) GLUT-4

d) Glucokinase

e) PFK-1

3) A 45-year-old obese man complains of having to get up frequently to urinate at night-time. He has also noticed that he is constantly thirsty and hungry. The patient is diagnosed with type 2 diabetes mellitus. If this is a problem at the level of glucose transporters , which tissue would be most affected?

a) Liver

b) Red blood cells

c) Muscle and adipose tissue

d) Brain tissue

e) Small intestine.

4) A 24-year woman is getting training for her first marathon. Her coach instructed her to keep a pace that allows her to stay below her anaerobic threshold. Under such conditions pyruvate does not accumulate as it is converted to-

a) Ethanol

b) Lactic acid

c) Acetyl co A

d) Alanine

e) Oxaloacetate.

5) A patient presents with dizziness, fatigue and tremors. A fingerstick test indicates a blood glucose level of 45 mg/dl (hypoglycemia). Glucagon is released under such condition . Which of the following allosteric modifier of PFK-1 would be  most effective in switching off glycolysis ?

a) Low level of citrate

b) Low level of Fr, 2,6 bisphosphate

c) Low level of ATP

d) Low level of NAD+

e) Low level of Glucose-6-P

6) A 30 -year-old woman is enduring her second trimester of pregnancy. The  greater affinity fetal hemoglobin acquires oxygen released from the lesser affinity maternal hemoglobin due to the effect of an allosteric modifier, which is-

a) Fr-2,6 bisphosphate

b) 2,3 bisphosphoglycerate

c) Citrate

d) Acetyl co A

e) Fr-1,6 bisphosphoglycerate

7) A pediatric hematologist sees an 18 month-old patient with jaundice, splenomegaly and hemolytic anemia. Blood smear indicates red blood cells that are more rigid in appearance than normal. A diagnosis of pyruvate kinase deficiency is made. What products would be made in low amounts ?

a) Acetyl co A

b) Glucose

c) Lactate

d) ATP

e) Alanine

8)  In an embryo with a complete deficiency of pyruvate kinase, how many net moles of ATP would be generated in the conversion of one mole of glucose to one mole of pyruvate ?

a) 0

b) 1

c) 2

d) 3

e) 4

9) Which out of the following enzymes cleaves a carbon-carbon bond in the pathway of glycolysis ?

a) Aldolase

b) Glyceraldehyde-3-P dehydrogenase

c) Enolase

d) Phosphoglycerate kinase

e) Phosphoglycerate mutase

10) After a heavy meal, which out of the following allosteric activator would be most effective in increasing the rate of glycolysis ?

a) ATP

b) Citrate

c) Acetyl co A

d) Fr-2,6 bisphosphate

e) 2,3 bisphosphoglycerate.

11) Compared with the resting state a vigorously contracting muscle shows-

a) An increased conversion of pyruvate to lactate

b) Increased oxidation of pyruvate to CO2

c) A decreased concentration of AMP

d) Decreased level of fr-2,6 bisphosphate

e) Decreased ratio of NADH/NAD+

12) A 24 -year-old man presented with symptoms of shortness of breath, weakness and fatigue. His Hemoglobin level was 7G/dl. Red blood cell isolated from the patient showed abnormally low-level of lactate. A deficiency of which of the following enzymes would be the most likely cause for patient’s anemia?

a) Phosphoglucose isomerase

b) Phosphofructokinase

c) Hexokinase

d) Glucokinase

e) Pyruvate kinase.

13) A mixture of sodium fluoride and potassium oxalate is added to sample collected for blood glucose estimation to prevent loss of glucose by anaerobic glycolysis. Fluoride is an inhibitor of glycolysis. Which of the following enzymes is inhibited by fluoride ?

a) Hexokinase

b) Phosphofructokinase

c) Glyceraldehyde-3-P dehydrogenase

d) Lactate dehydrogenase

e) Enolase

14) Oxamate is a competitive inhibitor of which of the following enzymes ?

a) Hexokinase

b) Phosphofructokinase

c) Glyceraldehyde-3-P dehydrogenase

d) Lactate dehydrogenase

e) Pyruvate dehydrogenase

15)  Which out of the following enzymes of glycolysis is inhibited by Arsenate ?

 a) Hexokinase

b) Phosphofructokinase

c) Glyceraldehyde-3-P dehydrogenase

d) Lactate dehydrogenase

e) Pyruvate dehydrogenase

Answers-

1)-e                2)-b                3)-c                 4)-c                 5)-b                6)-b              



7)-d                8)-a                9)-a                10)-d              11)-a              12)-e



13)-e              14)-d              15)-c



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