Tuesday, November 13, 2012

carbohydrate



1. Rotation of the plane of polarized light is caused by solutions of all of the following monosaccharides except
a. Glucose
b. Glyceraldehyde
c. Fructose
d. Dihydroxyacetone
e. None of the above

2. Ascorbic acid is an example of
a. Sugar acid
b. Sugar alcohol
c. Sugar phosphate
d. Deoxy-sugar
e. Amino sugar

3. An invert sugar is
a. An equimolar mixture of glucose and fructose
b. An equimolar mixture of a-glucose and 13-glucose
c. An equimolar mixture of a-fructose and 13-fructose
d. The sugar which changes its optical activity from levo- to dextrorotatory
e. None of the above

4. A disaccharide formed of two glucose units is
a. Lactose
b. Maltose
c. Sucrose
d. Amylose
e. Amylopectin

5. Milk sugar is
a. Cellobiose
b. Maltose
c. Sucrose
d. Lactose
e. None of the above

6. Cane sugar is
a. Cellobiose
b. Maltose
c. Sucrose
d. Lactose
e. None of the above

7. Malt sugar is
a. Cellobiose
b. Maltose
c. Sucrose
d. Lactose
e. None of the above

8. Starch is an example of
a. Galactosans
b. Mannosans
c. Glucosaminans
d. Glucosans
e. Fructosans

9. Glycogen is an example of
a. Glucosans
b. Galactosans
c. Mannosans
d. Glucosaminans
e. Fructosans

10. Starch is an example of
a. Structural polysaccharides present in animals
b. Structural polysaccharides present in plants
c. Nutrient polysaccharides present in animals
d. Nutrient polysaccharides present in plants
e. None of the above


11. Which of the following has a free anomeric carbon atom
a. Glucose
b. Mannose
c. Lactose
d. Sucrose
e. Fructose

12. A polysaccharide indigestible by man is
a. Cellobiose
b. Glycogen
c. Amylopectin
d. Cellulose
e. Amylose

13. A branch component of starch is
a. Glucose
b. Amylopectin
c. Amylose
d. Maltose
e. None of the above

14. Which of the following statements characterizes glucose
a. It usually exists in the furanose form
b. It is a ketose
c. Carbon 2 is the anomeric carbon atom
d. It forms part of the disaccharide sucrose
e. It is oxidized to form sorbitol

15. Which of the following contains ketone group
a. Ribulose
b. Glucose
c. Mannose
d. Galactose
e. Lactose

16. Which of the following is non fermentable sugar?
a. Maltose
b. Sucrose
c. Glucose
d. Fructose
e. Lactose

17. Hydrolysis of sucrose yields
a. Two moles of glucose
b. Glucose and fructose
c. Galactose and fructose
d. Glucose and mannose

18. Ribitol is
a. Deoxy sugar
b. Amino sugar
c. Sugar alcohol
d. Sugar acid

19. Inulin is a simple polysaccharide built up of
a. Glucosamine
b. Galactose
c. Fructose
d. Aldose

20. The end products of glycogen hydrolysis by acid is
a. Dextrin
b. Maltose
c. Amylose
d. Glucose

21. The richest site for fructose in the body is
a. Mammary gland
b. Seminal fluid
c. Thyroid gland
d. Prostate


22. D-glucose and D-mannose are epimers with respect to carbon atom number
a. 3
b. 2
c. 5
d. 1

23. Which of the following polysaccharides is not a polymer of glucose
a. Amylose
b. Glycogen
c. Inulin
d. Amylopectin

24. Reduction of glucose produces
a. Mannitol
b. Sorbitol
c. Dulcitol
d. Glucuronic acid

25. Gluconic acid, saccharic acid and glucuronic acid are produced from glucose by
a. Reduction
b. Treatment with acids
c. Oxidation
d. Treatment with bases

26. Galactose is present in the structure of
a. Sucrose
b. Lactose
c. Lecithin
d. Glycogen

27. Which statement is incorrect
a. Glucose and mannose are Epimers
b. α- and β-glucose are Anomers
c. Glucose and galactose are Anomers
d. Ribose and xylose are epimers

28. The invert sugar is
a. Maltose
b. Sucrose
c. Hydrolytic products of sucrose
d. Hydrolytic products of maltose

29. Sorbitol is
a. A sugar alcohol
b. Obtained from glucose
c. Obtained from fructose
d. All of the above

30. By complete acid hydrolysis of starch we obtain
a. Maltose
b. α-glucose
c. β-glucose
d. All of the above

31. The blood sugar is
a. Glucose
b. Fructose
c. Ribose
d. Galactose

32. Ribulose is an example of
a. Aldopentose
b. Ketohexose
c. Ketopentose
d. Deoxy sugar
e. Sugar alcohol

33. Cellulose is
a. Formed of β-glucose
b. Not digested in human intestine
c. A simple polysaccharide
d. All of the above

34. Dextrose is
a. An aldopentose
b. An aldohexose
c. A ketohexose
d. A ketopentose


35. Sorbitol can be obtained from fructose by
a. Oxidation
b. Reduction
c. Hydrolysis
d. Acetylation

36. A reducing disaccharide is
a. Glucose
b. Mannose
c. Maltose
d. Fructose

37. Glucose and mannose are epimers, this means that
a. They are mirror image to each other
b. One is aldose, the other is a ketose
c. They differ only in the configuration to one carbon
d. One is pyranose the other is furanose

38. Starch is
a. A heterogeneous polysaccharide
b. Composed of β-glucose
c. Not digested by amylase
d. All of the above
e. None of the above

39. The grape sugar is
a. Fructose
b. Sucrose
c. Glucose
d. Mannose

40. Lactose is
a. A non-reducing sugar
b. Formed of α-glucose and β-fructose
c. both (a) and (b)
d. None of the above

41. Honey is the natural example for
a. Sucrose
b. Maltose
c. Invert sugar
d. Lactose

42. Reducing property of monosaccharide is tested by
a. Seliwanoff's test
b. Molisch’s test
c. Fehling test
d. Rothera’s test
e. Shape of osazone crystals

43. Inulin is a polymer of
a. Glucose
b. Levulose
c. Galactose
d. Mannose

44. α.glucose is present in structure of
a. Dextrins
b. Dextran
c. Glycogen
d. All of the above

45. Amylase enzyme can hydrolyse
a. Starch
b. Dextrins
c. Glycogen
d. All of the above

46. Glucose and lactose are similar to each other in that both are
a. Reducing
b. Able to give the same osazone
c. Present in milk
d. All of the above


47. Which of the following is a disaccharide?
a. Starch
b. Cellulose
c. Glycogen
d. Ribose
e. Lactose

48. Which carbohydrate will you find in greatest abundance in potatoes?
a. Starch
b. Cellulose
c. Sucrose
d. Glycogen
e. Lactose

49. D-glucuronic acid is an example of
a. Aldonic acid
b. Aldaric acid
c. Uronic acid
d. Saccharic acid
e. None of the above

50. Reduction of monosaccharides yields
a. Sugar alcohols
b. Sugar acids
c. amino sugars
d. Deoxy-sugars
e. None of the above

51. Hydrogen gas in presence of a metal can reduce fructose to
a. Mannitol
b. Ribitol
c. Glycerol
d. Glucose
e. None of the above

52. Cellulose is made up of the molecules of
a. alpha Glucose
b. Beta Glucose
c. Both of above
d. None of the above

53. The epimer of glucose is:
a. Fructose
b. Galactose
c. Ribose
d. Deoxyribose

54. Alpha and Beta forms of D-glucose are referred to as:
a. Epimers
b. Anomers
c. Enediols
d. Tautomers

55. Which of the following does not give a positive test for a reducing sugar?
a. Ribose
b. Galactose
c. Maltose
d. Sucrose

56. Cellulose is a:
a. Branched polymer containing or 1 —> 4 and on 1 —> 6 glycosidic linkages
b. Straight chain polymer consisting of B 1 —> 4 glycosidic units
c. Polymer containing glucose, galactose and glucosamine
d. Mucopolysaccharide

57. In order that a compound possesses optical activity it must be:
a. Colored
b. Symmetrical
c. Inorganic
d. Asymmetric

58. Hydrolysis of sucrose yields:
a. Galactose and glucose
b. Maltose and glucose
c. Fructose only
d. Fructose and glucose


59. Starch and glycogen are both polymers of:
a. Fructose
b. Glucose-1-phosphate
c. Mannose
d. Glucose

60. Which of the following is not a CHO?
a. Amylose
b. Hyaluronic acid
c. Heparin
d. Palmitic acid

61. The predominant carbohydrate of muscle
a. D-fructose
b. D-glucose
c. Lactose
d. Glycogen

62. Lactose is also called:
a. Blood sugar
b. Invert sugar
c. Milk sugar
d. Animal starch

63. An example of pentose is:
a. Galactose
b. Mannose
c. Ribose
d. Fructose

64. An example of Hexose is:
a. Ribose
b. Ribulose
c. Xylulose
d. Mannose

65. A mucopolysaccharide with blood anticoagulant activity is:
a. Chondroitin sulphate B
b. Chondroitin sulphate D
c. Keratan sulphate II
d. Heparin

66. Beta 1-4 glycosidic bond is present in
a. Maltose
b. Lactose
c. Sucrose
d. None of the above

67. Number of stereo-isomers of glucose is :
a. 4
b. 8
c. 16
d. none of the above

68. A homopolysaccharide made up of fructose is :
a. inulin
b. dextrin
c. cellulose
d. glycogen

69. In fructofuranose ,the anomeric carbon is
a. carbon 1
b. carbon 2
c. carbon 3
d. carbon 4

70. A carbohydrate found in the DNA :
a. ribose
b. ribulose
c. deoxyribose
d. all of the above

71. Ribulose is a :
a. ketotetrose
b. aldotetrose
c. ketopentose
d. aldopentose


72. In D-glyecraldehyde, -OH group is present on the right hand side of carbon atom number :
a. 1
b. 2
c. 3
d. 1,2 & 3

73. A disaccharide made up of two glucose units :
a. maltose
b. sucrose
c. dextrin
d. lactose

74. A carbohydrate found only in milk :
a. glucose
b. lactose
c. galactose
d. maltose

75. A carbohydrate ,known commenly as invert sugar :
a. lactose
b. sucrose
c. fractose
d. glucose

76. The homopolysaccharide among the following is :
a. heparin
b. hyaluronic acid
c. dermatan sulphate
d. cellulose

77. The heteropolysaccharide among the following is :
a. inulin
b. starch
c. heparin
d. cellulose


78. In straight chain structure of D-glucose, -OH group is present on the left hand side of carbon atom number :
a. 2
b. 3
c. 4
d. 5

79. The following causes levorotation :
a. D-fructose
b. L-glucose
c. both of them
d. None of them

80. The carbon atom which becomes asymmetric when the straight chain form of monosaccharide changes into ring form is called :
a. anomeric carbon atom
b. epimeric carbon atom
c. isomeric carbon atom
d. none of the above


81. In straight chain structure of D-ribose, -OH group is present on the right hand side of carbon atom number :
a. 2
b. 3
c. 4
d. all of the above

82. In glucopyranose the anomeric carbon atom is number:
a. 2
b. 1
c. 5
d. 6


83. In alpha-D glucopyranose, -OH groups projecting below the plane of the ring, are attached to carbon atoms of numbers:
a. 1,2 & 3
b. 1,2 & 5
c. 1,2 & 4
d. 2,3 & 4

84. Sugar present in DNA is
a. ribose
b. xylulose
c. Arabinose
d. deoxyribose

85. Sugar present in RNA is:
a. Ribose
b. Xylulose
c. Arabinose
d. Deoxyribose

86. Sugar present in seminal fluid is:
a. glucose
b. fructose
c. galactose
d. maltose

87. All the following polysaccharides have glucose as their monomer EXCEPT
a. Starch
b. dextrin
c. Inulin
d. glycogen

88. End product of starch on acid hydrolysis is
a. glucose
b. fructose
c. maltose
d. dextrin

89. End product of enzyme hydrolysis of starch is
a. glucose
b. fructose
c. maltose
d. dextrin

90. Beta 1,4 glycosidic linkages are present in
a. Glycogen
b. starch
c. cellulose
d. inulin

91. All the following are homopolysaccharides EXCEPT
a. Cellulose
b. starch
c. glycogen
d. heparin

92. All the following are heteropolysaccharides EXCEPT
a. Hyaluronic acid
b. chondroitin sulphate
c. Inulin
d. Heparin

93. Which of the following poly saccharine is a naturally occurring anti coagulant?
a. Hylauronic acid
b. chondroitin sulphate
c. heparin
d. keratosulphate


94. One of the following carbohydrates is not digested in the human intestinal tract
a. Starch
b. cellulose
c. lactose
d. sucrose

95. Amylopectin differ from amaylose by
a. alpha 1,4 glycosidic linkage
b. beta 1,4 glycosidic linkage
c. alpha 1,4 glycosidic linkage at branching
d. alpha 1,6 glycosidic linkage at branching

96. One of the following polysaccharides is called animal starch:
a. glucose
b. heparin
c. dextrin
d. glycogen

97. Glucose on oxidation with H2O2 gives:
a. Hyaluronic acid
b. Glucuronic acid
c. Glucaric acid
d. Mucic acid

98. Sorbitol is:
a. A sterol
b. An amino alcohol
c. A sugar alcohol
d. A glycerol derivative

99. The functional group responsible for the reducing property of glucose is present on which carbon atom?
a. 6
b. 5
c. 2
d. 1

Answers
1. D
2. A
3. A
4. B
5. D
6. C
7. B
8. D
9. A
10. D
11. C
12. D
13. B
14. D
15. A
16. E
17. B
18. C
19. C
20. D
21. B
22. B
23. C
24. B
25. C
26. B
27. B
28. C
29. D
30. B
31. A
32. C
33. D
34. B
35. B
36. C
37. C
38. E
39. C
40. D
41. C
42. C
43. B
44. D
45. D
46. A
47. E
48. A
49. C
50. A
51. A
52. B
53. B
54. B
55. D
56. B
57. D
58. D
59. D
60. D
61. D
62. C
63. C
64. D
65. D
66. D
67. C
68. A
69. B
70. C
71. C
72. B
73. A
74. B
75. B
76. D
77. C
78. B
79. C
80. A
81. D
82. B
83. B
84. D
85. A
86. B
87. C
88. A
89. C
90. C
91. D
92. C
93. C
94. B
95. D
96. D
97. B
98. C
99. D



------------------------------------------ Best Wishes: Dr.Ehab Aboueladab, Tel:01007834123 Email:ehab10f@gmail.com,ehababoueladab@yahoo.com ------------------------------------------

Enzymes



1. Group of organic catalysts, protein in nature, present inside the living cells
a- Enzymes
b- Catalysts
c- Hormones
d- None of the above
2. All of the following is true regarding enzymes except …..
a- Heat labile
b- Colloidal
c- Dialyzable
d- High molecular weight
3. Organic substance on which the enzyme acts …….
a- Substrate
b- Co-Enzyme
c- Product
d- None of the above
4. All of the following can be considered as a co Enzyme for transfer of H except
a- Biotin
b- ATP
c- FAD
d- Folic acid
5. One of the following can be considered as a co Enzyme for transfer of groups other than H except
a- Biotin
b- ATP
c- FAD
d- Folic acid


6. One of the following is considered a feature of Enzymes except ……
a- Globular Proteins
b- Undergo Denaturation
c- Simple Proteins
d- Non-Specific
7. Enzyme acts on a special type of bond at specific site and attached to specific groups ……
a- Group Specificity
b- Absolute Specificity
c- Relative Specificity
d- Optical Specificity
8. D-amino acid Oxidase represents ……
a- Group Specificity
b- Absolute Specificity
c- Relative Specificity
d- Optical Specificity
9. Pancreatic Lipase represents …….
a- Group Specificity
b- Absolute Specificity
c- Relative Specificity
d- Optical Specificity

10. Special sequence of amino acids in the protein molecule of the enzyme to which the substrate is attached…….
a- Catalytic Site
b- Active site
c- Allosteric Site
d- None of the above


11. All sequences of amino acids which affect the activity of the enzyme ……
a- Catalytic Site
b- Active site
c- Allosteric Site
d- All of the above
12. Substrate induces a conformational change in the catalytic site …….
a- Lock and key model
b- Flexible model of catalytic site
c- Rigid model of catalytic site
d- None of the above
13. Concentration of the product increases directly by time …….
a- Maximum Velocity
b- End velocity
c- Initial Velocity
d- None of the above
14. Increase in substrate concentration leads to ……..
a- Increase of velocity
b- Increase in enzyme activity
c- Increase of V max
d- All of the above
15. Substrate concentration which produces half maximal velocity (½Vmax.) ……….
a- Full Concentration
b- Null Concentration
c- Michael’s Concentration
d- All of the above



16. Increase in enzyme concentration ……. the rate of reaction
a- Increase
b- Decrease
c- No effect
d- Permissive effect
17. Complete irreversible loss of enzyme activity occurs at ……
a- 37 degree C
b- 45 degree C
c- 0 degree C
d- 60-65 degree C
18. Temperature at which the enzymatic reaction velocity is maximal ……
a- Optimum Temperature
b- 37 degree C
c- 65 degree C
d- A and b together
e- None of the above
19. Optimum PH for Trypsin is ……
a- 6.8
b- 8.4
c- 8
d- 2
20. Optimum PH for Alkaline Phosphatase
a- 6.8
b- 8.4
c- 8
d- 2


21. All of the following inhibits enzymatic activity except
a- Physical Agents
b- Heating
c- Shaking
d- Red and Blue lights
22. Pepsin is activated through ……..
a- Removal of inhibitory peptide
b- Reducing agent
c- Minerals
d- Allosteric activators
23. Glyceraldehyde 3-P dehydrogenase is activated through …..
a- Removal of inhibitory peptide
b- Reducing agent
c- Minerals
d- Allosteric activators
24. Metaloenzymes is activated through …..
a- Removal of inhibitory peptide
b- Reducing agent
c- Minerals
d- Allosteric activators
25. Tyrosinase requires ……
a- ZN ++
b- CU ++
c- CL –
d- MG ++



26. Phosphofructokinase enzyme is activated through …..
a- Removal of inhibitory peptide
b- Reducing agent
c- Minerals
d- Allosteric activators
27. Activation by Phosphorylation excludes one of the following …….
a- Hormone sensitive lipase
b- Glycogen Phosphyrlase
c- Glycogen Synthetase
d- None of the above
28. Competitive inhibition includes all of the following except
a- Chemical structure of the inhibitor closely resembles that of the substrate.
b- Combines Reversibly with the enzyme
c- When both the substrate and the inhibitor are present they compete for the same binding site.
d- V max is decreased
e- Is removed by increases the concentration of inhibitor
29. Reversible noncompetitive Inhibition includes all of the following except
a- I combine with the enzyme away from the catalytic site.
b- The I is not similar to the substrate in structure.
c- The I can combine with the free enzyme or with the enzyme substrate
d- increase the Km
30. Phosphoglyceraldehyde dehydrogenase Resembles …….
a- Reversible Non Competitive Inhibition
b- Irreversible noncompetitive Inhibition
c- Allosteric inhibition
d- Competitive inhibition


31. Increased product concentration …… the enzyme activity
a- Increases
b- Decreases
c- No effect
d- Permissive effect
32. Substances which stimulate gene expression into protein …….
a- Inducer
b- Suppressor
c- Repressors
d- Co-Enzymes
33. Concerning Isoenzymes …….
a- Have quaternary structure and the individual subunits in each isoenzyme are different from the others
b- They act on the different substrate and will give the same product
c- They have different affinity to the substrate.
d- They are present in different tissues.
34. HMMM is present in ……..
a- Heart
b- Kidney
c- Liver
d- Muscles
35. HHMM is present in
a- Kidney
b- Muscles
c- Heart
d- None of the above



36. Non Functional plasma enzymes include all of the following except …..
a- Transaminases ( AST & ALT )
b- Alkaline Phosphatase
c- Lipoprotein lipase
d- None of the above
37. Increased in bone metastasis ………
a- Alkaline phosphatase
b- Creatine kinase
c- LDH
d- None of the above
38. Increased in Prostatic Carcinoma ………
a- Alkaline phosphatase
b- Creatine kinase
c- LDH
d- None of the above
39. Decreased in Galactosemia ………
a- Streptokinase
b- Digestive enzymes
c- Galactosyl transferase
d- α-chymotrypsin
40. Treatment of intraocular hemorrhage is done by
a- Streptokinase
b- Digestive enzymes
c- LDH
d- α-chymotrypsin
41. The oxidation process occurs by ………
a- Addition of oxygen.
b- Removal of hydrogen.
c- Loss of electron
d- All of the above
42. Redoxes that use oxygen as a hydrogen receptor are called ……..
a- Oxidases
b- Hyper peroxidases
c- Dehydrogenases
d- Oxygenases
43. Catalase is specifically abundant in all of the following except ……..
a- Liver
b- Kidney
c- Erythrocytes
d- Milk and leucocytes
44. Enzymes utilizing H2O2 as substrate ……….
a- Oxidases
b- Hyper peroxidases
c- Dehydrogenases
d- Oxygenases

45. LDH depends on ………. In dehydrogenation
a- Nicotinamide
b- FAD
c- C.AMP
d- None of the above
46. Enzymes which catalyze transfer of functional groups (G) other than hydrogen between a pair of substrates …….
a- Transferase
b- Oxygenases
c- Dehydrogenases
d- Hydrolases

47. All of the following are included in Transferase class of enzymes except …….
a- Transaminase
b- Hydrolase
c- Transglycosayl
d- None of the above
48. Split terminal peptide linkage splitting one amino acid at a time is ……..
a- Estrases
b- Endoeptidases
c- Exopeptidases
d- Glycosidases
49. Fumerase is an example of …….
a- Transferase
b- Hydrolase
c- Oxidase
d- Lyases
50. Lipase is an example of …….
a- Transferase
b- Estrases
c- Oxidases
d- Lyases
51. Enzymes link two molecules using energy from ATP ……
a- Lyases
b- Hydrolase
c- Ligase
d- Redox
52. Carboxylase is an example of …….
a- Transferase
b- Estrases
c- Oxidases
d- Ligases
Choose (A) for true and (B) For false of the following
53. Pepsin acts on peptide bonds between amino groups of aromatic amino acid and carboxylic group of another amino acid
54. Enzymes are usually specific in action
55. In Absolute specificity the enzyme acts at different rates on one type of bond in
Compounds chemically related
56. Enzymes may be considered to lower energy barriers for chemical reactions
57. Increase in the substrate concentration will lead to decrease in enzyme activity
58. Increase in enzyme concentration increase the rate of reaction
59. Some enzymes containing SH groups requires Minerals to be activated
60. In Competitive inhibition Vmax of the enzyme is not decreased
61. Inducers are substances which inhibit gene expression
62. Isoenzyme is oligomeric enzyme

Answers
1. A
2. C
3. A
4. C
5. C
6. D
7. A
8. D
9. C
10. A
11. B
12. B
13. C
14. A
15. C
16. A
17. D
18. D
19. C
20. B
21. D
22. A
23. B
24. C
25. B
26. D
27. C
28. D
29. D
30. A
31. B
32. A
33. B
34. D
35. A
36. A
37. A
38. B
39. C
40. D
41. D
42. A
43. D
44. B
45. A
46. A
47. B
48. C
49. D
50. B
51. C
52. D
53. A
54. A
55. B
56. A
57. B
58. A
59. B
60. A
61. B
62. A







------------------------------------------ Best Wishes: Dr.Ehab Aboueladab, Tel:01007834123 Email:ehab10f@gmail.com,ehababoueladab@yahoo.com ------------------------------------------

hemoglobin


Q. What are the salient structural features of hemoglobin molecule?
A. Hb has four subunits, two alpha and two beta
units. It contains four iron atoms.

Q. How many molecules of oxygen can bind with hemoglobin?
A. Hb can bind four molecules of oxygen.

Q. 100 ml of blood can carry how much oxygen?
A. 20 ml.

Q. What is Bohr effect? 
A. The influence of pH and pCO2 to facilitate oxygenation of Hb in the lungs and deoxygenation
at the tissues is known as the Bohr effect.

Q. What is chloride shift?
A. When CO2 is taken up, chloride ions from the plasma would enter. This is called chloride shift
or Hamburger effect.

Q. Hemoglobin carries how much CO2? 
A. Seventy five percent as isohydric transport and 15% as carbamino hemoglobin.

Q. What are the forms in which carbon dioxide is transported? 
A. Dissolved form, isohydric transport, and carbamino hemoglobin.

Q. What is isohydric transport of carbon dioxide?
A. There is minimum change in pH during the transport.

Q. How this is effected? 
A. The H+ ions are buffered by the deoxy-Hb.

Q. Which will decrease the affinity of hemoglobin for oxygen?
A. 2,3-BPG level.

Q. What is the structural difference between HbA and HbF?
A. HbA has two alpha and two beta chains, but HbF has two alpha and two gamma chains.

Q. What are the laboratory tests to identify HbF?
A. HbF moves slower than HbA on electrophoresis, HbF is alkali resistant.

Q. What is the physiological significance of HbF?
A. Oxygen affinity is more for HbF than HbA. HbF is seen in foetal circulation.


Q. What is the clinical significance of HbF?
A. It is seen in adults in hemoglobinopathies and thalassemias.

Q. Why carbon monoxide becomes a poison?
A. Hb has more affinity to carbon monoxide than oxygen.

Q. What is the treatment for carbon monoxide poison? 
A. Hyperbaric oxygen.

Q. Met-hemoglobin is found in which conditions?
A. Ingestion of nitrites, presence of HbM, GPD deficiency.

Q. What is met-hemoglobin? 
A. Hemoglobin in which iron is in ferric state.

Q. What is the defect of met-hemoglobin?
A. It cannot release oxygen in tissues.

Q. What is the reagent used for colourimetric estimation of hemoglobin? 
A. Drabkin’s reagent, to convert Hb to cyanmethemoglobin.

Q. What is hemoglobin S? 
A. The glutamic acid in the 6th position of beta chain of HbA is changed to valine in HbS.


Q. What is the cause for sickle cell anemia?
A. Solubility of deoxy HbS is lower than deoxy HbA,so HbS is precipitated intracellularly, leading to
sickle shape of RBC.

Q. How HbS is identified?
A. HbS is slower moving on electrophoresis than HbA.

Q. What is sickle cell trait?
A. Heterozygous (AS) condition, one allele is normal, the other is abnormal, so half of Hb molecules are normal, and half abnormal.

Q. What is its clinical significance?
A. Sickle cell trait persons will not have any disease manifestations, usually. But, at higher altitudes,
hypoxia may cause manifestation of the disease.Chronic lung disorders may also produce
hypoxia-induced sickling in HbS trait.


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Water soluble vitamins


Q. What is the source of thiamine? 
A. Aleurone layer of cereals (food grains) is a rich source of thiamine. Whole wheat flour and
unpolished rice and yeast are very good sources.

Q. Thiamine pyrophosphate is required for which reactions ? 
A. Transketolase, pyruvate dehydrogenase, and alpha keto glutarate dehydrogenase.

Q. Which Vitamin is required for oxidative decarboxylation? 
A. Thiamine pyrophosphate.

Q. In thiamine deficiency, what alterations are seen in blood ? 
A. Increased pyruvic acid level and increased transketolase activity.

Q. What are the clinical manifestations of thiamine
deficiency?
A. Beriberi, Wernick’s syndrome, and polyneuritis.

Q. Beberi is due to the deficiency of which Vitamin?
A. Thiamine.

Q. What is the daily requirement of Vitamin B1 (thiamine) ? 
A. 1 to 1.5 milligram.

Q. Chronic alcoholism may lead to the deficiency of which Vitamin?
A. Vitamin B1.

Q. Wernicke’s encephalopathy is due to the deficiency of which Vitamin?
A. Vitamin B1.

Q. What is the co-enzyme function of riboflavin ?
A. FMN and FAD dependent enzymes.

Q. What are the FAD dependent enzymes?
A. Succinate dehydrogenase, acyl CoA dehydrogenase,xanthine oxidase, glutathione reductase,
glycine cleavage system, pyruvate dehydrogenase,and alpha ketoglutarate dehydrogenase.

Q. What are the manifestations of riboflavin deficiency? 
A. Glossitis, cheilosis, angular stomatits, andcircumcorneal vascularisation.

Q. What is the dietary sources of riboflavin?
A. Rich sources are liver, dried yeast, egg, and milk.

Q. What is the daily requirement of riboflavin?
A. 1.5 mg per day.

Q. Which Vitamin is synthesised in the body?
A. Niacin.

Q. What is the co-enzyme function of niacin?
A. NAD and NADP.

Q. Name some important NAD dependent enzymes.
A. Lactate dehydrogenase, glyceraldehyde-3-phosphate dehydrogenase, pyruvate dehydrogenase,
beta hydroxy acyl CoA dehydrogenase,and mitochondrial isocitrate dehydrogenase.

Q. Name the NADPH generating reactions.
A. Glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase, malic enzyme, and
cytoplasmic isocitrate dehydrogenase.

Q. What are the important NADPH utilising reactions? 
A. Beta keto acyl ACP dehydrogenase, alpha, betaunsaturated ACP dehydrogenase, HMGCoA
reductase, met-hemoglobin reductase, dihydrofolatere ductase, and phenylalanine hydroxylase.

Q. Pellagra is seen in the deficiency of which Vitamin? 
A. Niacin.

Q. What are the features of pellagra ? 
A. Dermatitis, diarrhea, and dementia.

Q. What is the precursor of niacin? 
A. Tryptophan.

Q. Tryptophan will give rise to how much niacin?
A. About 60 mg of tryptophan will yield 1 mg of niacin.

Q. Tryptophan is deficient in which food stuff?
A. Maize and corn.

Q. Which conditions will lead to symptoms of pellagra? 
A. Isoniazid therapy, low tryptophan content in diet,niacin deficiency, hartnup disease, and carcinoid
syndrome.

Q. What is the dietary sources of niacin?
A. Natural sources of niacin are dried yeast, rice polishing,liver, peanut, whole cereals, legumes, meat and fish. About half of the requirement is met by the conversion of tryptophan to niacin. About 60 mg of tryptophan will yield 1 mg of niacin.

Q. What is the daily requirement of niacin?
A. The R.D.A is 20 mg/day.

Q. Transamination reaction requires which Vitamin? 
A. Pyridoxal phosphate.

Q. Pyridoxal phosphate is required for what reactions? 
A. Transamination reactions, decarboxylation of amino acids, ALA synthase, glycogen phosphorylase.

Q. Give an example of transamination reaction.
A. Alanine transaminase (ALT) will catalyse the reaction, alanine to pyruvate.

Q. What is its clinical significance?
A. Blood level of ALT is increased in liver diseases,AST is increased in myocardial infarction.

Q. Give some examples of decarboxylation reactions.
A. Glutamate to GABA (gamma aminobutyric acid),histidine to histamine, 5-hydroxy tryptophan to
serotonin, cysteine to taurine, and serine to ethanol amine.

Q. Xanthurenic acid in urine is seen in the deficiency of which Vitamin?
A. Pyridoxal deficiency.

Q. What are the manifestations of pyridoxal deficiency? 
A. Infantile convulsions, peripheral neuritis,pellagra, and anemia.

Q. What is the reason for infantile convulsions in pyridoxal deficiency?
A. Pyridoxal phosphate is required for the formation of GABA, and it is an inhibitory neurotransmitter,
absence of which leads to hyper-excitation
and convulsions.

Q. What is the reason for peripheral neuritis in pyridoxal deficiency?
A. PLP is involved in the synthesis of sphingolipids,so B6 deficiency leads to demyelination of nerves
and consequent peripheral neuritis.

Q. What is the reason for pellagra-like disease in pyridoxal deficiency?
A. Since niacin is produced from tryptophan, one of the enzymes of this pathway is PLP dependent.
So B6 deficiency in turn leads to niacin deficiency which is manifested as pellagra.

Q. Can you give an exmple of one Vitamin deficiency leading to another Vitamin deficiency?
A. PLP deficiency in turn leads to niacin deficiency which is manifested as pellagra.

Q. What is the reason for anemia in pyridoxal deficiency? 
A. PLP is required for ALA synthase. In adults hypochromic microcytic anemia may occur due
to the inhibition of heme biosynthesis.

Q. What are the drugs which cause pyridoxal deficiency? 
A. Isonicotinic acid hydrazide (INH) (isoniazid),cycloserine, penicillamine and oral contraceptives
act as B6 antagonists. Ethanol in the body is converted to acetaldehyde, which inactivates PLP.

Q. What are the dietary sources of pyridoxal?
A. Rich sources are yeast, rice polishing, wheat germs, cereals, legumes (pulses), egg, milk, meat,
fish and green leafy vegetables.

Q. What is the daily requirement of pyridoxal phosphate? 
A. 1 to 2 mg/day.

Q. What is the co-enzyme form of pantothenic acid?
A. Co-enzyme A.

Q. What is the function of CoA? 
A. Oxidation of pyruvic acid and activation of acylgroups.

Q. Deficiency of pantothenic acid leads to what?
A. Burning foot syndrome.

Q. What are the donors for acetyl CoA pool?
A. Pyruvate, fatty acid, and amino acids.

Q. Acetyl CoA is used for what purposes?
A. Oxidation in TCA cycle, fatty acid synthesis,cholesterol synthesis, steroid synthesis, and
ketone body formation.

Q. Succinyl CoA is used for what purposes?
A. Oxidation in TCA cycle, heme synthesis, and activation of acetoacetate.

Q. What is the function of biotin? 
A. Carboxylation reactions.

Q. Name some reactions dependent on biotin.
A. Acetyl CoA carboxylase, propionyl CoA carboxylase, and pyruvate carboxylase.

Q. What is the antagonist for biotin? 
A. Avidin.

Q. What is its use in laboratory? 
A. Biotin-avidin reaction is used in immunosorbent assays.

Q. What is the chemical nature of folic acid?
A. Pteroyl glutamic acid (pteridine + PABA + glutamic acid).

Q. What is PABA?
A. Para amino benzoic acid.

Q. What is the co-enzyme form of folic acid?
A. Tetrahydro folic acid.

Q. What is the main function of folic acid?
A. Tetrahydro folic acid is the carrier of one carbon units.

Q. What are the causes of folate deficiency?
A. Pregnancy, defective absorption (sprue, celiac disease), anticonvulsant drugs (hydantoin,
dilantin, phenytoin, phenobarbitone), hemolytic anemias, and dietary deficiency.

Q. What is the major manifestation of folic acid deficiency?
A. Macrocytic anemia.

Q. Folic acid deficiency in pregnancy is associated with what? 
A. Folic acid deficiency during pregnancy may lead to neural tube defects in the fetus. Folic acid
prevents birth defects (fetal malformations such as spina bifida).

Q. What are the other minor effects of folic acid?
A. Folic acid is also useful to reduce the level of homo-cysteine in blood, and therefore helps in
preventing heart diseases. Folic acid is beneficial in prevention of cancer.

Q. What are the laboratory findings in folic acid deficiency? 
A. (1) Normal folic acid level in serum is decreased.(2) FIGLU is excretion is more, especially after
histidine load. (3) AICAR excretion in urine. (4) Peripheral blood picture shows macrocytic anemia. (5)
Homocysteine level in blood is increased

Q. What are the sources of folic acid? 
A. Yeast, liver, egg, green leafy vegetables, cereals,and pulses.

Q. What is the daily requirement of folic acid?
A. The RDA of free folate is 200 mg/day. In pregnancy the requirement is increased to 400 mg/day.

Q. What is the mechanism of action of sulphonamides?
A. They are anti-bacterial agents. They have structural similarity with PABA. Therefore they competitively
inhibit the enzyme responsible for the incorporation of PABA into folic acid.

Q. What is the mechanism of action of trimethoprim?
A. It inhibits the folate reductase and so formation of THFA is reduced. It is bactericidal agent.

Q. What is mechanism of action of methotrexate?
A. It inhibits folate reductase, and is a powerful anticancer drug.

Q. What are inhibitors of folic acid? 
A. Methotrexate, trimethoprim, pyrimethamine and sulphonamide.

Q. Name a water soluble Vitamin, which is stored in the body.
A. Vitamin B12.

Q. What is the metal present in Vitamin B12?
A. Cobalt.

Q. What is the ring system present in Vitamin B 12?
A. Corrin ring.

Q. Vitamin B12 is absorbed from where?
A. Ileum.

Q. What is the transport form of Vitamin B12?
A. Methyl B 12.

Q. What is the carrier of Vitamin B12 in blood?
A. Transcobalamin-II, a glycoprotein, is the specific carrier.

Q. What is the storage form of Vitamin B12?
A. It is stored in the liver cells, as ado-B12 form, in combination with transcobalamin-I or transcorrin.

Q. What is the co-enzyme function of Vitamin B 12?
A. Methyl malonyl CoA isomerase, and homocysteine methyl transferase.

Q. What abnormalities are seen in Vitamin B 12 deficiency? 
A. Methyl malonic aciduria, accumulation of methylmalonic acid, breakdown of myelin sheaths and
interruption in nerve transmission. Homocysteinuria is also seen.

Q. What abnormal compound is excreted in Vitamin B12 deficiency?
A. Methyl malonic acid in urine.

Q. What is folate trap? 
A. The production of methyl THFA is an irreversible step. Therefore, the only way for generation of free
THFA is methyl THFA to THFA, by a Vitamin B12 dependent step. When B12 is deficient, this
reaction cannot take place. This is called the methyl folate trap.

Q. What is the clinical importance of folate trap?
A. This leads to the associated folic acid scarcity in B12 deficiency.

Q. What is the explanation of demyelination in Vitamin B12 deficiency?
A. In Vitamin B12 deficiency, there is non-availability of active methionine. Therefore, methylation
of phosphatidyl ethanolamine to phosphatidylcholine is not adequate. This leads to
deficient formation of myelin sheaths of nerves.

Q. What are the causes of B12 deficiency?
A. Nutritional B12 deficiency, decrease in absorption,addisonian pernicious anemia, atrophy of
gastric epithelium, and pregnancy.

Q. What is the cause for pernicious anemia?
A. It is an autoimmune disease with a strong familial background. Antibodies are generated against
intrinsic factor. So IF is deficient, leading to defective absorption of B12.

Q. What are the manifestations of Vitamin B12 deficiency?
A. Megaloblastic anemia and subacute combined egeneration.

Q. What is the difference in folate deficiency and B12 deficiency?
A. In folate deficiency, there is macrocytic anemia,and in B12 deficiency, there is additional
neurological symptoms also.

Q. What is the daily requirement of Vitamin B12?
A. One to two microgram/per day.

Q. A patient who has undergone gastrectomy is likely to develop deficiency of which Vitamin?
A. Vitamin B12.

Q. In Vitamin B12 deficiency, what are the abnormalities seen in urine?
A. Urine may contain methyl malonic acid, homocystine,cystathione, and formimino glutamic acid.

Q. Which is the Vitamin totally absent in plant sources?
A. Vitamin B12.

Q. What are the sources of Vitamin B12?
A. Liver, meat, fish, and curd.

Q. What is the chemical structure of Vitamin C?
A. L-ascorbic acid.

Q. How it is synthesised? 
A. Man and primates cannot synthesise ascorbic acids. Lower animals could synthesise it from
glucose through glucuronic acid pathway.

Q. What are the major functions of ascorbic acid?
A. Ascorbic acid promotes collagen formation through its action on post-translational
hydroxylation of proline and lysine residues.

Q. What are the other functions of ascorbic acid?
A. Parahydroxy phenyl pyruvate oxidation to homogentisic acid, iron absorption from the intestine,
reconversion of methemoglobin to hemoglobin,and adrenal steroidogenesis.

Q. Which Vitamin is required for post-translational modifications?
A. Ascorbic acid.

Q. What is the daily requirement of ascorbic acid?
A. 75 mg per day.

Q. Scurvy is due to the deficiency of which Vitamin?
A. Ascorbic acid.

Q. What are the important features of scurvy?
A. Hemorrhagic tendency, microcytic anemia, bone pain, bleeding gums


------------------------------------------ Best Wishes: Dr.Ehab Aboueladab, Tel:01007834123 Email:ehab10f@gmail.com,ehababoueladab@yahoo.com ------------------------------------------